如何计算Spectrophotometry
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Spectrophotometry measures light absorption: A = log(I₀/I) = εbc (Beer-Lambert law), used for quantitative analysis.
分步指南
- 1Input measured absorbance, path length, molar absorptivity
- 2Calculate concentration
- 3Generate calibration curve if multiple samples
例题解析
输入
Absorbance 0.5, ε = 5000 M⁻¹cm⁻¹, b = 1 cm
结果
c = A/(εb) = 0.5/5000 = 1×10⁻⁴ M
常见错误注意事项
- ✕Using wrong wavelength for compound
- ✕Not accounting for baseline absorbance
常见问题
What's molar absorptivity?
Characteristic of molecule at specific wavelength; tabulated values for known compounds.
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